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Oocyte Cryopreservation: A 9-Year Single-Centre Experience

Published:October 19, 2022DOI:https://doi.org/10.1016/j.jogc.2022.10.006

      Abstract

      Objective

      Oocyte cryopreservation (OC) has increased in recent years; however, there is a paucity of published data on the use of cryopreserved oocytes and associated outcomes.

      Methods

      A retrospective review of 748 OC cycles between 2013 and 2022 at a private fertility centre was performed. Outcome parameters for oocyte retrieval cycles were reviewed. For patients who returned for oocyte disposition, outcomes subsequent to oocyte re-warming, fertilization, and transfer were analyzed.

      Results

      There were 748 OC cycles (653 elective and 95 non-elective) in 646 patients (556 elective and 90 non-elective). Patients were older at the time of freezing in the elective oocyte group compared with the non-elective group (36.5 vs. 28.8 y; P < 0.001). Sixty-five patients returned to warm and fertilize their oocytes (50 in the elective group and 15 in the non-elective group). The survival rate for warmed oocytes was 76.1% (541/711), and 66.2% of surviving oocytes were successfully fertilized, and 39.1% reached blastulation. Twenty-three patients underwent embryo transfers (10 after preimplantation genetic testing for aneuploidy), with 15 patients having at least 1 delivery or ongoing pregnancy.

      Conclusions

      To date, this is the largest published experience with OC in Canada. OC can lead to successful live births but does not guarantee a viable outcome for all patients. In this study, most patients with vitrified oocytes had not returned for disposition, so long-term follow-up is still required to verify the efficacy of OC.

      Résumé

      Objectif

      La cryoconservation d’ovocytes (CO) a gagné en popularité dans les dernières années; cependant, il y a très peu de données publiées sur l’utilisation des ovocytes cryoconservés et les résultats qui en découlent.

      Méthodologie

      Une étude rétrospective de 748 cycles de CO entre 2013 et 2022 a été menée dans une clinique de fertilité privée. Les critères de jugement pour les cycles de prélèvement d’ovocytes ont été examinés. Pour les patientes qui sont revenues à la clinique pour utiliser les ovocytes, les résultats subséquents au réchauffement des ovocytes, à la fécondation et au transfert d’embryons ont été analysés.

      Résultats

      Il y a eu 748 cycles d’OC (653 pour préservation de la fertilité et 95 pour assistance médicale) chez 646 patientes (556 pour préservation de la fertilité et 90 pour assistance médicale). Les patientes étaient plus âgées au moment de la cryoconservation dans le groupe préservation que dans le groupe médical (36,5 p/r à 28,8 ans; p < 0,001). Au total, 65 patientes sont revenues pour le réchauffement et la fécondation de leurs ovocytes (50 dans le groupe préservation et 15 dans le groupe médical). Le taux de survie des ovocytes réchauffés était de 76,1 % (541/711), et 66,2 % des ovocytes survivants ont été fécondés avec succès, dont 39,1 % ont atteint le stade de blastulation. En tout, 23 patientes ont subi un transfert d’embryon (10 après un dépistage génétique préimplantatoire des aneuploïdies); de ce nombre, 15 ont connu au moins un accouchement ou une grossesse établie.

      Conclusions

      À ce jour, il s’agit de la plus grande expérience publiée sur la CO au Canada. La CO peut mener à des naissances vivantes, mais ne garantit pas un résultat de grossesse viable pour toutes les patientes. Dans cette étude, la plupart des patientes ayant des ovocytes vitrifiés ne les ont toujours pas utilisés, de sorte qu’un suivi à long terme demeure nécessaire pour vérifier l’efficacité de la CO.

      Keywords

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