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The High Prevalence of Vitamin D Insufficiency in Cord Blood in Calgary, Alberta (APrON-D Study)

Published:March 21, 2017DOI:https://doi.org/10.1016/j.jogc.2017.01.008

      Abstract

      Objective

      Vitamin D is important in promoting healthy pregnancy and fetal development. We undertook this study to measure 25-hydroxyvitamin D in maternal and cord blood and to identify maternal factors related to vitamin D status in Calgary.

      Methods

      Blood samples collected at the time of delivery from the Alberta Pregnancy Outcomes and Nutrition study cohort (ApronStudy.ca) participants were processed for plasma and assayed using liquid chromatography mass spectrometry methodology for 25(OH)D3.

      Results

      Ninety-two pairs of maternal and cord blood samples were obtained. The prevalence of 25(OH)D3 insufficiency—25(OH)D3 <75 nmol/L—was 38% and 80% in women and neonates, respectively. Vitamin D supplementation was the only clinical factor associated with 25(OH)D3 sufficiency, and the odds of sufficiency were 3.75 (95% CI 1.00 to 14.07) higher for women and 5.27 (95% CI 1.37 to 20.27) when over 2000 IU/day were used.

      Conclusion

      Using liquid chromatography mass spectrometry, we demonstrated a very high prevalence of vitamin D insufficiency in cord blood and that the use of high dose vitamin D was associated with greater odds of sufficiency in pregnant women and cord blood in Alberta.

      Résumé

      Objectif

      La vitamine D favorise la santé durant la grossesse et joue un rôle important dans le développement fœtal. Nous avons entrepris cette étude afin de mesurer la quantité de 25-hydroxyvitamine D présente dans le sang maternel et le sang de cordon et de mettre en évidence les facteurs maternels influant sur l'état nutritionnel en vitamine D des femmes enceintes de Calgary.

      Méthodologie

      Des échantillons de sang ont été prélevés lors de l'accouchement de mères participant à l'étude Alberta Pregnancy Outcomes and Nutrition (apronstudy.ca). Le plasma a été extrait de ces échantillons, puis la quantité de vitamine D (25-OH-D3) a été mesurée par chromatographie en phase liquide couplée à la spectrométrie de masse.

      Résultats

      Quatre-vingt-douze paires d'échantillons de sang maternel et de sang de cordon ont été prélevées. La prévalence de l'insuffisance en 25-OH-D3 – concentration de 25-OH-D3<75 nmol/L – était de 38 % chez les mères et de 80 % chez les nouveau-nés. Le seul facteur clinique associé à un taux suffisant de 25-OH-D3 était la prise de suppléments de vitamine D. Il était 3,75 fois plus probable que le taux de 25-OH-D3 soit suffisant chez les femmes prenant des suppléments que chez celles qui n'en prenaient pas (IC à 95 % : 1-14,07), et 5,27 fois plus probable que le taux soit suffisant (IC à 95 % : 1,37-20,27) si la dose de suppléments était supérieure à 2 000 UI/jour.

      Conclusions

      Au moyen de l'analyse des échantillons sanguins par chromatographie en phase liquide couplée à la spectrométrie de masse, nous avons démontré que la prévalence de l'insuffisance en vitamine D dans le sang de cordon est très élevée et que la prise de doses élevées de suppléments de vitamine D est associée à une probabilité accrue que cette dernière soit présente en quantité suffisante dans le sang de cordon et dans le sang des femmes enceintes en Alberta.

      Key Words

      Abbreviations:

      APrON (Alberta Pregnancy Outcomes and Nutrition), LC-MS/MS (liquid chromatography mass spectrometry)
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